|
KMID : 0363620100310040020
|
|
Journal of Korean Oriental Medicine
2010 Volume.31 No. 4 p.20 ~ p.37
|
|
|
Effect of YCT on Insulin Secretion in RIN-m5F Cells
|
|
Kim Jin-Mi
Cho Chung-Sik
Kim Chul-Jung
|
|
|
Abstract
|
|
|
Objective: This study was performed to investigate the effect of Yagwan-cheunghyeoltang (YCT) on insulin secretion in RIN-m5F cells.
Methods: After treatment with various concentrations of YCT to RIN-m5F cells, cell viability, free radical-scavenging activity, SOD activity, and insulin secretion were measured. Additionally, insulin-related gene expressions were measured using real-time RT-PCR.
Results: 1. YCT didn’t show any influence on RIN-m5F cells viability. 2. YCT showed free radical-scavenging activity by 16% at of concentration. 3. YCT showed enhancement of SOD activity by 60% at of concentration. 4. YCT significantly increased insulin secretion in RIN-m5F cells in a dose-dependent manner. 5. YCT up-regulated INS-1, INS-2, IRS-1, IRS-2 and IRS-3 mRNA expressions compared to the control group. 6. YCT down-regulated INS-R, GCK, GLP-1R and GLP-2R mRNA expressions compared to the control group.
Conclusion: YCT has pharmaceutical properties enhancing insulin production and controlling glucose-associated metabolism, and could be a candidate for drug development after further research.
|
|
|
KEYWORD
|
|
|
Yagwan-cheunghyeoltang(YCT), RIN-m5F, Insulin secretion, Gene expression
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|